An algorithm for assessment of inflow and washout of optical contrast agent to the brain by analysis of time-resolved diffuse reflectance and fluorescence signals

In optical measurements of the brain oxygenation and perfusion the problem of contamination of the signals with the components related to the extracerebral tissues remains an obstacle limiting clinical applicability of the technique. In this paper we present an algorithm allowing for derivation of signals related to the changes in absorption in the intracerebral tissues based on analysis of time-resolved diffuse reflectance and fluorescence. The proposed method was validated in series of Monte Carlo simulations in which inflow and washout of an optical contrast agent into the two-layered human head model was considered. It was shown that the decomposed intracerebral component of the signal can be derived with uncertainty of about 5%. This result suggests that the method proposed can be applied in improved estimation of brain perfusion parameters based on the bolus-tracking technique.