Title :
Expression of c-erbB-2 in breast cancer cell lines as experimental receptor of magnetic nanoparticles
Author :
Silva, Jesus G. ; Sanchez, Victor ; Polo, Salvador M. ; Gonzalez, Camila A.
Author_Institution :
EMGS-UDEFA, México City, Mexico
Abstract :
High mortality in breast cancer is associated to a late diagnosis and therapy of the disease. Our research group is working on the development of an innovative technology to promote selective ablation of breast cancer in early stages by the use of high frequency magnetic fields assisted by magnetic nanoparticles. The concept behind the technical proposal is to increase the electrical conductivity of tumoral tissue by the use of bioconjugated “magnetic nanoparticle-monoclonal antibody”. Is expected that bioconjugated recognizes specific genes on the surface of cancer cells. The aim of this study was to evaluate the expression of the c-erbB-2 gene and c-erbB-2 antigen in breast cancer cells type BT-474, MCF-7 and MDA-MB-231, as previous step to propose the c-erbB-2 protein as receptor of magnetic nanoparticles. The results suggest that the elected breast cancer cell lines show well-differentiated relative expression of the elected gen and antigen, and sems suitable for experimental evaluation of selective targeting by magnetic nanoparticles.
Keywords :
biomagnetism; biomolecular effects of radiation; cancer; cellular effects of radiation; electrical conductivity; genetics; magnetic particles; nanomedicine; nanoparticles; proteins; radiation therapy; tumours; BT-474; MCF-7; MDA-MB-231; bioconjugated magnetic nanoparticle-monoclonal antibody; breast cancer cell line; c-erbB-2 antigen expression; c-erbB-2 gene expression; c-erbB-2 protein; cancer cell surface; disease therapy; elected gen; electrical conductivity; high frequency magnetic field; late diagnosis; magnetic nanoparticle receptor; selective ablation; selective targeting; sems; specific gene recognition; tumoral tissue; Breast cancer; Conductivity; Magnetic fields; Magnetic resonance imaging; Medical treatment; Nanoparticles;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE
Conference_Location :
Osaka
DOI :
10.1109/EMBC.2013.6610546