DocumentCode :
638818
Title :
Normalization of flow-in velocity for improving the evaluation on cell deformability
Author :
Tsai, Chia-Hung Dylan ; Sakuma, Shinya ; Arai, Fumihito ; Kaneko, Makoto
Author_Institution :
Dept. of Mech. Eng., Osaka Univ., Suita, Japan
fYear :
2013
fDate :
4-7 Aug. 2013
Firstpage :
261
Lastpage :
266
Abstract :
A method for improving the evaluation of cell deformability is proposed. Cell deformability can be evaluated by the ratio between the cell velocities before and after entering the narrow throat of a microchannel. However, the flow-in velocity, the cell velocity before entering the throat, varies with respect to the location of the cell because of the laminar flow inside the microchannel. To compensate such velocity variation caused by different locations, we obtain a mean velocity by normalizing the velocity profile, and utilize this mean velocity for evaluating cell deformability. Experimental study on human red blood cells is performed. By implementing a high-speed vision system, we acquire the information of the location and velocity of every single cell through the narrow throat. The acquired data are processed by the proposed method. We compare both the results with and without the normalization, and find that the the proposed method effectively improve the consistency and accuracy of the evaluation.
Keywords :
biomechanics; blood; cellular biophysics; data acquisition; deformation; image sensors; laminar flow; microchannel flow; cell deformability evaluation; cell velocity; data acquisition; data processing; flow-in velocity; flow-in velocity normalization; high-speed vision system; human red blood cells; laminar flow; microchannel; narrow throat entering; velocity profile; velocity variation; Accuracy; Diseases; Force; Machine vision; Microchannel; Trajectory; Velocity measurement;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Mechatronics and Automation (ICMA), 2013 IEEE International Conference on
Conference_Location :
Takamatsu
Print_ISBN :
978-1-4673-5557-5
Type :
conf
DOI :
10.1109/ICMA.2013.6617928
Filename :
6617928
Link To Document :
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