Raman and Surface-Enhanced Raman Scattering (SERS) Studies of the Thrombin-Binding Aptamer

Author

Tsai-Chin Wu ; Vasudev, M. ; Dutta, Maitreyee ; Stroscio, Michael

Author_Institution

Bioeng. Dept., Univ. of Illinois, Chicago, IL, USA

Volume

12

Issue

2

fYear

2013

fDate

Jun-13

Firstpage

93

Lastpage

97

Abstract

Surface-enhanced Raman scattering is used to study the Raman spectra and peak shifts the thrombin-binding aptamer (TBA) on substrates having two different geometries; one with a single stranded sequence and one with double stranded sequence. The Raman signals of the deoxyribonucleic acids on both substrates are enhanced and specific peaks of bases are identified. These results are highly reproducible and have promising applications in low cost nucleic acid detection.

Keywords

DNA; biochemistry; biological techniques; enzymes; molecular biophysics; molecular configurations; surface enhanced Raman scattering; Raman signals; Raman spectra; deoxyribonucleic acids; double stranded sequence; low cost nucleic acid detection; molecular geometry; peak shifts; single stranded sequence; substrates; surface-enhanced Raman scattering study; thrombin-binding aptamer; Aptamer; DNA; nanobiotechnology; nanoparticle; surface-enhanced Raman scattering; Aptamers, Nucleotide; DNA; Gold; Metal Nanoparticles; Potassium; Spectrum Analysis, Raman;

fLanguage

English

Journal_Title

NanoBioscience, IEEE Transactions on

Publisher

ieee

ISSN

1536-1241

Type

jour

DOI

10.1109/TNB.2013.2242484

Filename

6516949