Microfluidic biochip for phytoplankton cell counting

Author

Matiolli Machado, Leandro ; Mattiello, Izadora ; Mendes Lopes, Rubens ; Carreno, Marcelo Nelson Paez

Author_Institution

Lab. de Microeletronica, USP, São Paulo, Brazil

fYear

2013

fDate

2-6 Sept. 2013

Firstpage

1

Lastpage

4

Abstract

We present a prototype of a PDMS-based microfluidic device for automatic counting of phytoplankton cells in culture solution. The device functioning is based on monitoring changes in the intensity of a cross-channel fiber optical signal during downstream cell transit. The microfluidc chip is fabricated by PDMS soft lithography technique, and the light source and the detection unit are commercial LED and photodiodes integrated to the microfluidic device. The results demonstrate the reliability of the automatic counting experiments as compared with a manual microscopic counting method.

Keywords

bioMEMS; biological techniques; botany; cellular biophysics; lab-on-a-chip; light emitting diodes; light sources; microfabrication; microfluidics; optical fibres; photodiodes; soft lithography; PDMS soft lithography technique; PDMS-based microfluidic device; automatic counting; commercial LED; cross-channel fiber optical signal intensity; culture solution; detection unit; device functioning; downstream cell transit; light source; manual microscopic counting method; microfluidic biochip; photodiodes; phytoplankton cell counting; Microfluidics; automatic phytoplankton counting; biochips;

fLanguage

English

Publisher

ieee

Conference_Titel

Microelectronics Technology and Devices (SBMicro), 2013 Symposium on

Conference_Location

Curitiba

Print_ISBN

978-1-4799-0516-4

Type

conf

DOI

10.1109/SBMicro.2013.6676146

Filename

6676146