On-chip base sequencing using a two-stage reaction-control scheme: 3.6-times-faster and 1/100-reduced-data-volume ISFET-based DNA sequencer

Author

Yanagawa, Yukiko ; Itabashi, Naoki ; Migitaka, S. ; Yokoi, Takahide ; Yoshida, Manabu ; Kawahara, Toshio

Author_Institution

Central Res. Lab., Hitachi, Ltd., Kokubunji, Japan

fYear

2013

fDate

Oct. 31 2013-Nov. 2 2013

Firstpage

178

Lastpage

181

Abstract

A novel two-stage reaction-control scheme with a background-cancelling circuit - for reducing data volume and analysis time of an ion-sensitive-FET (ISFET) - based DNA sequencer-is proposed. The scheme successfully reduces the background noise and renders time-consuming background analysis unnecessary. It also reduces sample-to-data time by 72% (3.6 times faster) and data volume by two orders of magnitude.

Keywords

DNA; biochemistry; biological techniques; data analysis; ion sensitive field effect transistors; lab-on-a-chip; molecular biophysics; noise; background noise reduction; background-cancelling circuit; data analysis time reduction; data volume reduction; ion-sensitive-field effect transistors; on-chip base sequencing; reduced-data-volume ISFET-based DNA sequencer; two-stage reaction-control scheme; Arrays; DNA; Heating; Polymers; Sequential analysis; System-on-chip; Transistors; DNA sequencer; Ion-sensitive FET; on-chip base-sequencing;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Circuits and Systems Conference (BioCAS), 2013 IEEE

Conference_Location

Rotterdam

Type

conf

DOI

10.1109/BioCAS.2013.6679668

Filename

6679668