Title :
Raman mapping studies of human ectocervical tissue
Author :
Daniel, Andy ; Arunal, P. ; Ganesan, S. ; Koteeswaran, D. ; Murali Krishna, C. ; David, Barak
Author_Institution :
Dept. of Med. Phys., Anna Univ., Chennai, India
Abstract :
In this pilot study, the micro Raman mapping of normal cervical tissue was carried out to understand the biomolecular alteration at different regions of the cervical tissue. The constructed Raman mapping was compared with respect to different layers of cervical tissue viz., Superficial layer, Intermediate layer, Parabasal layer, Basal layer and underlying Connective tissue through the averaged Raman spectrum at the respective sites. Raman spectra collected from the basal side of epithelium consistently showed higher DNA contributions and lower lipid contributions when compared with superficial epithelium spectra. Spectra of the underlying connective tissue exhibited a strong contribution of collagen and elastin fibrils.
Keywords :
DNA; Raman spectra; Raman spectroscopy; biological tissues; molecular biophysics; patient diagnosis; proteins; Basal layer; DNA contribution; Intermediate layer; Parabasal layer; Raman mapping studies; Superficial layer; averaged Raman spectrum; biomolecular alteration; collagen; connective tissue; elastin fibrils; epithelium basal side; human ectocervical tissue; lipid contribution; microRaman mapping; normal cervical tissue; superficial epithelium spectra; Pathology; Raman spectroscopy; Uterine cervix; microspectrocopic mapping;
Conference_Titel :
Photonics (ICP), 2013 IEEE 4th International Conference on
Conference_Location :
Melaka
Print_ISBN :
978-1-4673-6073-9
DOI :
10.1109/ICP.2013.6687082
