Title :
High throughput low cost electrochemical device for S.aureus bacteria detection
Author :
Safavieh, Mohammadali ; Ahmed, Mobyen Uddin ; Zourob, Mohammed
Author_Institution :
Energy Mater., Telecommun., Univ. du Quebec, Varennes, QC, Canada
Abstract :
We present a novel roll-to-roll point of care (POC) diagnostic device that is easy to use, low cost to prepare, high throughput and can detect nucleic acids electrochemically in a real time manner. We first, demonstrate fabrication of the flexible ribbon. Then, the preparation of the device has been presented. The device has been used for detection of the real sample of S.aureus bacteria following by real time electrochemical detection. We have shown that we could detect 200 CFU/ml bacteria in 40 min using LAMP amplification technique.
Keywords :
electrochemical sensors; macromolecules; microorganisms; rolling; LAMP amplification technique; POC; S.aureus bacteria detection; electrochemical detection device; flexible ribbon; nucleic acid detection; roll-to-roll point of care diagnostic device; time 40 min; DNA; Electrodes; Isothermal processes; Microorganisms; Real-time systems; Reservoirs; Substrates;
Conference_Titel :
SENSORS, 2013 IEEE
Conference_Location :
Baltimore, MD
DOI :
10.1109/ICSENS.2013.6688159