Glucose sensing and driven “organic engine” with co-immobilized enzyme membrane for actuation by blood sugar in diabetes

In order to develop chemo-mechanical autonomous drug release system which is able to be driven by low glucose concentration close to blood sugar level for diabetic treatment, the organic engine with co-immobilized enzyme membrane was developed and tested. The enzymes (glucose oxidase (GOD), pyranose oxidase (POX), alcohol oxidase (AOX), galactose oxidase (GAO)) that oxidize glucose and/or glucono-1.5-lactone were evaluated for co-immobilization and, the most effective decompression was obtained in the organic engine with co-immobilized POX002B;GOD membrane. As a result, the decompression rate in the organic engine with POX002B;GOD membrane was increased 3 times higher than the conventional one with GOD membrane, and necessary decompression in the organic engine for driving the drug release system was obtained at 10 mmol/L glucose which is close to the blood sugar level. Therefore, the organic engine with co-immobilized enzyme membrane showed a promising result for development of chemo-mechanical system actuated by human blood sugar for diabetic treatment.