Mammary cancer cell manipulation with embedded passivated-electrode insulator-based dielctrophoresis (EπDEP)

Author

Shake, Thomas ; Srinivasaraghavan, V. ; Zellner, Phillip ; Agah, Masoud

Author_Institution

Bradley Dept. of Electr. & Comput. Eng., Virginia Tech, Blacksburg, VA, USA

fYear

2013

fDate

3-6 Nov. 2013

Firstpage

1

Lastpage

4

Abstract

In this paper, we introduce a new embedded passivated-electrode insulator-based dielectrophoresis (EπDEP) device for cell manipulation. This device maximizes the electric field strength in the microfluidic channel by reducing the thickness of the passivation layer to 5μm. The devices are made by polymer molding using 3D glass molds fabricated by melting glass into features created by the RIE-lag technique on silicon. This paper demonstrates the trapping of MDA-MB-468 mammary cancer cells using EπDEP technology with very high efficiency (97%).

Keywords

bioelectric potentials; biomedical electrodes; cancer; cellular biophysics; electrophoresis; glass; melting; microchannel flow; moulding; passivation; polymers; sputter etching; 3D glass molds; EπDEP technology; MDA-MB-468 mammary cancer cells; RIE-lag technique; Si; SiO₂; electric field strength; embedded passivated-electrode insulator-based dielectrophoresis; glass melting; mammary cancer cell manipulation; microfluidic channel; passivation layer thickness; polymer molding; silicon surface; Cancer; Charge carrier processes; Dielectrophoresis; Electric fields; Electrodes; Glass; Silicon;

fLanguage

English

Publisher

ieee

Conference_Titel

SENSORS, 2013 IEEE

Conference_Location

Baltimore, MD

ISSN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2013.6688314

Filename

6688314