Temperature-controlled electrochemical microwell platform for studying biomolecular interactions

We designed and fabricated, on a 4-inch wafer, prototype devices for proof-of-concept studies on biomolecular interactions. The platform includes eight probe sites, each consisting of one 200 nm thick platinum (Pt) heater, one 200 nm gold (Au) and two 200 nm Pt electrodes, as well as a 20 μL polydimethylsiloxane (PDMS) well for electrochemical probing of samples. The Pt heaters have the ability to increase sample temperatures up to 120 ^oC. The device can perform electrochemical binding based measurements that detect changes in methylene blue (MB)-labeled DNA attached on the Au electrodes.