Title :
HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions
Author :
Mambo, Masahiro ; Jonathan, Okonkwo O. ; Nana, Agyei M.
Author_Institution :
Dept. of Environ., Water, & Earth Sci., Tshwane Univ. of Technol., Pretoria, South Africa
Abstract :
The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. Using Cd2002B; as a model divalent metal ion, the inhibition rate was proportional to the concentration in the range from 0.002-0.030 mg L-1 with a limit of detection of 0.51 μg L-1. Representative Dixon and Cornish-Bowden plots showed that the reaction was reversible and noncompetitive.
Keywords :
amperometric sensors; biosensors; carbon nanotubes; composite materials; electrochemical electrodes; electrostatic devices; organic compounds; C; Cornish-Bowden plot; Dixon plot; FTIR; HRP biosensor; MT-MWCNT composite; UV-Vis; amperometric biosensor; carbonized maize tassel- MWNT modified electrode; divalent trace metal ion detection; electrochemical behavior; electrostatic interaction; horseradish peroxidase activity; maize tassel-multiwalled carbon nanotube; Biochemistry; Biosensors; Electrodes; Films; Ions; Metals; Sugar; Amperometric biosensor; Heavy metal; Horseradish peroxidase; Inhibition; Maize tassel; Multi walled carbon nanotubes;
Conference_Titel :
SENSORS, 2013 IEEE
Conference_Location :
Baltimore, MD
DOI :
10.1109/ICSENS.2013.6688375