Application of chemiluminescence involving flow injection analysis to determination of glucose, glutamic acid and lactic acid for food analysis

A simple application of chemiluminescence (CL) to multicomponent analysis of glucose, glutamic acid and lactic acid for food analysis using flow injection analysis (FIA) is described. The oxidative enzyme for each analyte and horseradish peroxidase (HRP) was employed. The sample solutions were analysed by the catalytic reaction on luminol/H₂O₂ CL reaction system. The CL generated by catalytic reactions was detected by a FIA system consisted of a photodiode, enzyme-immobilized reactor, a peristaltic pump and a personal computer. Glucose oxidase (GOD), glutamate oxidase (GLOD) and lactate oxidase (LOD) was immobilized on the hydrophilic polytetrafluoroethylene membrane, respectively. A small volume, 5.0 μL of the standard solution of each analyte was injected and the CL was detected and measured by a photodiode. And the measurement of glucose, glutamic acid and lactic acid is concurrently also possible by using GOD, GLOD and LOD enzyme-immobilized reactors.