Anisotropic bacterial adsorbed micro chambers by microfluidic trapping array

Bacteria based biomedical micro-robot is being investigated or proposed to achieve effective and localized drug delivery system due to low toxicity and free of external power sources. In this work, we proposed a simple microfluidic system that enables anisotropic bacterial attachment to the drug containing micro chamber made of biodegradable polymer. A pin-ball type trapper can capture only one micro chamber at a fixed configuration. The efficiency of the trapper was analyzed by monitoring the motion of chambers. More than 70% of trapper was filled with the micro chamber within seconds, leading high throughput applications. We are planning to introduce modified Salmonella bacteria for absorbing them to the specific sides of the micro chamber. After releasing the chamber, one would use it as a microrobot by the chemotaxis of the attached bacteria towards the targeted site.