Title :
New facile method for preparing themperature-resopnsive cell culture surfaces using a thioxantone-based photoinitiator immobilized polystyrene surface
Author :
Fukumori, Kazuhiro ; Tanaka, Nobuyuki ; Ota, Hiroyuki ; Akiyama, Yoko ; Itoga, Kazuyoshi ; Yamato, Masayuki ; Okano, Teruo
Author_Institution :
Instn. for Adv. Biomed. Sci., Tokyo Women´s Med. Univ., Tokyo, Japan
Abstract :
PIPAAm grafted polystyrene (PIPAAm-Pst) dishes were prepared using visible light irradiation and polystyrene dishes modified with thioxanethone-based photoinitiator. At optimized preparation conditions, PIPAAm-PSs fabricating cell sheets were successfully prepared by 5 mins visible light irradiation. After PIPAAm polymerization, micro-patterned temperature responsive polystyrene culture surface (MPTRCS) were generated by additional micro-patterned polymerization of PAAm. Endothelial cells were attached selectively PIPAAm region at 37°C. By lowing temperature, micro-pattered endothelial cells were detached from MPTRCS as continuously micro-pattered cell sheet.
Keywords :
biochemistry; cellular biophysics; materials preparation; polymer blends; polymerisation; radiation effects; MPTRCS; PIPAAm grafted polystyrene; PIPAAm-PS fabricating cell sheets; micropatterned PIPAAm polymerization; micropatterned endothelial cell sheet; micropatterned temperature responsive polystyrene culture surface; optimized preparation conditions; polystyrene dishes; temperature 37 degC; themperature-resopnsive cell culture surfaces; thioxanethone-based photoinitiator; thioxantone-based photoinitiator immobilized polystyrene surface; time 5 min; visible light irradiation; Biomedical imaging; Bovine; Fibroblasts; Polymers; Radiation effects; Solvents; Surface treatment;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-1527-9
DOI :
10.1109/MHS.2013.6710390
