Title :
Single influenza virus infection reveals the difference between G1 and S/G2/M cell
Author :
Honda, A. ; Sugiura, Toshihiko ; Kume, Shinichiro ; Hiramatsu, Hidenori ; Suzuki, Yuya ; Nagatsuka, Yasuko ; Hirabayashi, Yuji ; Arai, Manabu ; Arai, Fumihito
Author_Institution :
Dept. of Frontier Biosci., Hosei Univ., Koganei, Japan
Abstract :
Using optical tweezers for trapping and infection of influenza virus influenza virus binds selectively G1 phase cell. This result encouraged us to dissolve the difference between G1- and S/G2/M-phage cells. At the beginning, we tried to analyze the sialic acid content of both G1-and S/G2/M-phase cells. Because it had been shown that influenza virus attached to the sialic acid on the cell membrane. Obviously the sialic acid content was different between both cells. Next the membrane stiffness and lipid composition were analyzed. The result of membrane stiffness showed that the membrane stiffness of G1-phase cell was increased than S/G2/M-phase cell. The result of lipid composition assay showed that the expression of Gb3, Gb4, and GlcCer was higher in S/G2/M-phase cells than in G1-phase cells.
Keywords :
biomechanics; biomembranes; cellular biophysics; elastic constants; lipid bilayers; microorganisms; radiation pressure; G1 phase cell; Gb3 expression; Gb4 expression; GlcCer expression; S-G2-M-phase cell; cell membrane; lipid composition; membrane stiffness; optical trapping; optical tweezers; sialic acid content; single influenza virus infection; Biomembranes; Computer architecture; Influenza; Lipidomics; Microprocessors; Optical buffering;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-1527-9
DOI :
10.1109/MHS.2013.6710428
