Title :
Microfluidics facilitated genome sequencing for limited number of cells
Author :
Liang Zhao ; Xiannian Zhang ; Streets, Aaron M. ; Yuhong Pang ; Fuchou Tang ; Yanyi Huang
Author_Institution :
Biodynamic Opt. Imaging Center (BIOPIC), Peking Univ., Beijing, China
Abstract :
We develop a microfluidics-based single cell RNA-Seq transcriptome analysis technology to perform the library-prep reaction steps at nanoliter range within sealed chambers on-chip, eliminating potential contaminations and sophisticated manual handlings.
Keywords :
RNA; bioMEMS; biochemistry; cellular biophysics; genomics; lab-on-a-chip; microfabrication; microfluidics; chamber on-chip; genome sequencing; library-prep reaction steps; microfluidics; single-cell RNA-sequencing transcriptome analysis technology; Bioinformatics; Biomedical imaging; Genomics; Libraries; Microfluidics; Performance evaluation; System-on-chip;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
