Title :
Highly sensitive optofluidic FRET lasers with genetically encoded fluorescent protein pairs
Author :
Qiushu Chen ; Xingwang Zhang ; Sun, Yue ; Ritt, Marcus ; Sivaramakrishnan, Sriram ; Xudong Fan
Author_Institution :
Dept. of Biomed. Eng., Univ. of Michigan, Ann Arbor, MI, USA
Abstract :
We achieved optofluidic FRET lasers using genetically encoded fluorescent protein pairs linked by length-tunable peptides. 10-fold enhancement in the FRET sensing signal was demonstrated with the optofluidic FRET laser compared to the conventional FRET detection.
Keywords :
biomedical measurement; fluorescence; genetics; laser applications in medicine; optical sensors; proteins; 10-fold enhancement; FRET sensing signal; conventional FRET detection; genetically encoded fluorescent protein pairs; highly sensitive optofluidic FRET lasers; length-tunable peptides; Fluorescence; Lasers; Optical ring resonators; Optical sensors; Protein engineering; Proteins;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
