DocumentCode
689253
Title
Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope
Author
Lew, Matthew D. ; Backlund, Mikael P. ; Backer, Adam S. ; Sahl, Steffen J. ; Grover, Ginni ; Agrawal, Ankit ; Piestun, Rafael ; Moerner, W.E.
Author_Institution
Dept. of Chem., Stanford Univ., Stanford, CA, USA
fYear
2013
fDate
9-14 June 2013
Firstpage
1
Lastpage
3
Abstract
Ignoring the anisotropic nature of single-fluorophore emission in super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.
Keywords
fluorescence; optical microscopy; position measurement; 3D molecule orientation measurement; 3D molecule position measurement; double-helix microscope; single-fluorophore emission; super-resolution microscopy; DH-HEMTs; Microscopy; Optical microscopy; Position measurement; Spatial resolution; Three-dimensional displays;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location
San Jose, CA
Type
conf
Filename
6833636
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