Title :
Measuring the 3D position and orientation of single molecules simultaneously and accurately with the double helix microscope
Author :
Lew, Matthew D. ; Backlund, Mikael P. ; Backer, Adam S. ; Sahl, Steffen J. ; Grover, Ginni ; Agrawal, Ankit ; Piestun, Rafael ; Moerner, W.E.
Author_Institution :
Dept. of Chem., Stanford Univ., Stanford, CA, USA
Abstract :
Ignoring the anisotropic nature of single-fluorophore emission in super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.
Keywords :
fluorescence; optical microscopy; position measurement; 3D molecule orientation measurement; 3D molecule position measurement; double-helix microscope; single-fluorophore emission; super-resolution microscopy; DH-HEMTs; Microscopy; Optical microscopy; Position measurement; Spatial resolution; Three-dimensional displays;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
