Title :
Non-blinking "Giant" nanocrystal quantum dots: Ideal probes for real-time three-dimensional tracking
Author :
Hollingsworth, Jennifer A. ; Werner, J.H. ; Han Htoon ; Piryatinski, Andrei ; Schaller, Richard ; Ghosh, Yagnaseni ; Dennis, Allison M. ; Keller, Aaron M. ; Mangum, Benjamin ; Hannah, Daniel C.
Author_Institution :
Mater. Phys. & Applic., Los Alamos Nat. Lab., Los Alamos, NM, USA
Abstract :
We describe the development and characterization of two novel non-blinking “giant” nanocrystal quantum dot (g-NQD) systems and their application to single-particle tracking in live cells real-time and in three dimensions. The two g-NQDs - CdSe/CdS and InP/CdS - are characterized by a thick shell layer (>10 monolayers of CdS), a quasi-type-II or type-II electronic structure, and extremely suppressed non-radiative Auger recombination and photobleaching, in addition to suppressed blinking. We show that these unique traits make this new class of NQD ideal molecular probes for observing spatio-temporal dynamics of cellular processes.
Keywords :
Auger effect; II-VI semiconductors; III-V semiconductors; biological techniques; cadmium compounds; cellular biophysics; electron-hole recombination; indium compounds; molecular biophysics; nanophotonics; optical saturable absorption; semiconductor quantum dots; spatiotemporal phenomena; CdS monolayers; CdSe-CdS; InP-CdS; NQD ideal molecular probes; cellular processes; g-NQD; ideal probes; live cell real-time; nonblinking giant nanocrystal quantum dot system characterization; nonblinking giant nanocrystal quantum dot system development; nonradiative Auger recombination; photobleaching; quasi-type-II electronic structure; real-time three-dimensional tracking; single-particle tracking; spatio-temporal dynamics; suppressed blinking; thick shell layer; Fluorescence; Indium phosphide; Nanocrystals; Photonic band gap; Radiative recombination; Three-dimensional displays;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
