Title :
Magnetic particle spectroscopy of glioma cells targeted with lactoferrin-conjugated iron oxide nanoparticle contrast agents
Author :
Gandhi, Sonu ; Tomitaka, Asahi ; Arami, Hamed ; Krishnan, Kannan M.
Author_Institution :
Dept. of Mater. Sci. & Eng., Univ. of Washington, Seattle, WA, USA
Abstract :
MPI is a real time imaging modality that promises micromolar sensitivity together with sub-mm spatial resolution using iron oxide nanoparticle tracers [1]. Here, the potential of lactoferrin conjugated iron oxide nanoparticles (Lf-IONPs) in oncology as contrast agents [2] for targeted magnetic particle imaging [3] was explored in glioma cells in vitro. We demonstrated receptor-mediated uptake by C6 glioma cells with Prussian blue staining (PBS) and confocal laser scanning microscopy (CLSM), and evaluated the efficiency of these nanoparticles as MPI contrast agents for cancer diagnosis.
Keywords :
biomagnetism; biomedical imaging; cancer; cellular biophysics; dyes; iron compounds; magnetic particles; nanocomposites; nanomagnetics; nanomedicine; nanoparticles; optical microscopy; tumours; C6 glioma cell; Fe2O3; Prussian blue staining; cancer diagnosis; confocal laser scanning microscopy; iron oxide nanoparticle tracers; lactoferrin-conjugated iron oxide nanoparticle contrast agents; magnetic particle spectroscopy; micromolar sensitivity; oncology; real time imaging modality; receptor-mediated uptake; spatial resolution; targeted magnetic particle imaging; Cancer; Imaging; In vitro; Iron; Magnetic particles; Nanoparticles; Spectroscopy;
Conference_Titel :
Magnetic Particle Imaging (IWMPI), 2015 5th International Workshop on
Conference_Location :
Istanbul
Print_ISBN :
978-1-4799-7269-2
DOI :
10.1109/IWMPI.2015.7107002