Development of in situ microbial ATP analyzer and internal standard calibration method

Author

Hanatani, Kohei ; Fujii, Teruo ; Fukuba, Tatsuhiro

Author_Institution

Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan

fYear

2015

fDate

23-25 Feb. 2015

Firstpage

1

Lastpage

4

Abstract

Integrated and miniaturized in situ analyzers to estimate microbial biomass has been developed so far. The analyzer has the capability to perform the luciferin-luciferase bioluminescence reaction to determine the concentration of microbial adenosine-5´-triphosphate (ATP). The latest analyzer had been mounted on an ROV and evaluated at deep-sea environments. In this study, the method for in situ calibration that utilizes caged ATP as an internal standard is proposed to improve reliability of the in situ analyzer. Experiments confirmed that caged ATP was photolyzed with appropriate reproducibility by using a simple microfluidic device with the UV-LED light source.

Keywords

bioMEMS; biochemistry; biological techniques; bioluminescence; biosensors; calibration; cellular biophysics; chemical sensors; enzymes; light emitting diodes; measurement standards; microfluidics; microorganisms; molecular biophysics; oceanographic equipment; optical sensors; photolysis; remotely operated vehicles; spectrochemical analysis; ROV; UV-LED light source; adenosine-5´-triphosphate; analyzer evaluation; caged ATP photolysis reproducibility; deep-sea environment; in situ analyzer reliability; in situ calibration; in situ microbial ATP analyzer development; integrated in situ analyzer; internal standard calibration method development; luciferin-luciferase bioluminescence reaction; microbial ATP concentration determination; microbial biomass estimation; microfluidic device; miniaturized in situ analyzer; Calibration; Electron tubes; Light emitting diodes; Plastics; ATP; biomass; calibration; microbe; microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Underwater Technology (UT), 2015 IEEE

Conference_Location

Chennai

Print_ISBN

978-1-4799-8299-8

Type

conf

DOI

10.1109/UT.2015.7108276

Filename

7108276