Extensions of spatiotemporal image correlation spectroscopy

One dimensional spatiotemporal image correlation spectroscopy (1D-STICS), a confocal microscopy technique for quantifying flow velocities, was evaluated over a large range of velocities, fluorophore sizes, and fluorophore concentrations to determine the practical working range and guidelines for implementation. We have demonstrated high spatial sensitivity for flow profile mapping and improved processing algorithms to make it feasible to analyze temporally changing flow velocities. Additionally, we are extending the use of 2D-STICS beyond the intra-cellular scale, to the higher speeds and size-scale of musculoskeletal tissues to map dynamic fluid processes.