Implementation of high-performance LSM using wavelet transformation analysis

Laser scanning microscopy (LSM), especially the laser scanning electron microscopy (LSCM) has become a technique which has been frequently used to acquire images of living cells with high resolution and contrast ratio in biomedical researchs including clinical biology. LSCM stands out as one of the most powerful microscopy techniques compared to conventional microscopy methods due to its capability of acquiring slices in the 3rd dimension by solely imaging the cell layers that lies only on focal plane. This study aims to increase the resolution of images by reducing the SNR of data which is obtained from resolution target by established confocal microscopy setup and subsequently using wavelet transform analysis.