Detection and counting of Oncorhynchus Mykiss spermatozoa

Today in the world, a large portion of the trout species grown in the fisheries constitutes Oncorhynchus Mykiss. In order to increase the efficiency of Oncorhynchus Mykiss´s production, it is expected to be in fair value of its sperm quality parameters for fertilize. Investigation by examination under a microscope with the conventional method of quality parameters is the waste of time for researchers. In this work, we present a system that can detect the cell concentration, which is an important quality parameter for the fish spermatozoa, with Otsu Threshold and Connected Component Labeling methods.