Biological particle manipulation: An example of Jurkat enrichment

Author

Altinagac, Emre ; Ozcan, Safiye Selen ; Genc, Yavuz ; Kizil, Huseyin ; Trabzon, Levent

Author_Institution

Dept. of Nanosci. & Nanoengineering, Istanbul Tech. Univ., Istanbul, Turkey

fYear

2015

fDate

7-11 April 2015

Firstpage

25

Lastpage

27

Abstract

Separation of microparticles and living cells are studied with computational and experimental methods. The flow behaviors of particles are modeled and simulated using COMSOL Multiphysics 4.3a software. Lab-on-a-chip devices having a Ti interdigitated electrode layer on a glass substrate and a PDMS microchannel are fabricated to investigate the most effective design for separating particles based on their sizes. Polystyrene particles in different diameters (3.2 μm and 9.8 μm) are used in our experiments and experiments with T-lymphocytes and B lymphoblasts are in progress.

Keywords

bioMEMS; cellular transport; lab-on-a-chip; microchannel flow; microfabrication; polymers; separation; titanium; B lymphoblasts; COMSOL Multiphysics 4.3a software; Jurkat enrichment; PDMS microchannel; T-lymphocytes; Ti interdigitated electrode layer; biological particle manipulation; flow behaviors; glass substrate; lab-on-a-chip devices; living cells; microparticle separation; polystyrene particles; size 3.2 mum; size 9.8 mum; Biology; Dielectrophoresis; Electric fields; Electrodes; Force; Microchannels; Microfluidics; AC Dielectrophoresis; Lab-on-a-Chip; Microfluidics; Particle Separation;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2015 IEEE 10th International Conference on

Conference_Location

Xi´an

Type

conf

DOI

10.1109/NEMS.2015.7147348

Filename

7147348