An ELISA-based amperometric biosensor within a photo-patternable adhesive microfluidic channel

Author

Yan Li ; Van Roy, Willem ; Vereecken, Philippe M. ; Lagae, Liesbet

Author_Institution

IMEC, Leuven, Belgium

fYear

2015

fDate

7-11 April 2015

Firstpage

76

Lastpage

81

Abstract

We present an amperometric biosensor enhanced by redox cycling under laminar flow at microelectrodes. The device integrates the enzyme-linked immunosorbent assay and the interdigitated electrode array within a photo-patternable adhesive microfluidic channel. The adhesive enables us to perform high quality bioassay within a microfluidic channel. Laminar flow enhances the mass transport not only from enzymatic region to the redox cycling electrodes, but also between the redox cycling electrodes. As a result, the performance of biosensors is enhanced.

Keywords

amperometric sensors; bioMEMS; biochemistry; biosensors; enzymes; laminar flow; mass transfer; microchannel flow; microelectrodes; microsensors; oxidation; reduction (chemical); ELISA-based amperometric biosensor; enzymatic region; enzyme-linked immunosorbent assay; high quality bioassay; interdigitated electrode array; laminar flow; mass transport; microelectrode; photopatternable adhesive microfluidic channel; redox cycling electrodes; Biochemistry; Biosensors; Current measurement; Electric potential; Electrodes; Gold; Microfluidics; amperometric biosensor; convective redox cycling; microfluidics; photo-patternable adhesive;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2015 IEEE 10th International Conference on

Conference_Location

Xi´an

Type

conf

DOI

10.1109/NEMS.2015.7147377

Filename

7147377