Immunochromatographic Assay of Hepatitis B Surface Antigen Using Magnetic Nanoparticles as Signal Materials

Author

Kyuha Oh ; Young Soo Choi ; Ha Young Yoon ; Nami Park ; Jooho Kim ; Young Keun Kim

Author_Institution

R&D Center, Bio Focus Co., Ltd., Uiwang, South Korea

Volume

50

Issue

11

fYear

2014

fDate

Nov. 2014

Firstpage

1

Lastpage

4

Abstract

An immunochromatographic assay for the detection of hepatitis B surface antigen (HBsAg) was developed using Fe₃O₄ nanoparticles as signal materials. Anti-HBsAg antibodies were labeled with carboxylated Fe₃O₄ particles with an average diameter of 153.6 nm, and an immunochromatography strip was constructed. The detection capability of the assay developed here is 1 ng/mL HBsAg.

Keywords

biochemistry; biomagnetism; chemical sensors; chromatography; diseases; iron compounds; magnetic particles; molecular biophysics; nanomedicine; nanoparticles; nanosensors; proteins; Fe₃O₄; HBsAg; anti-HBsAg antibodies; detection capability; hepatitis B surface antigen; immunochromatographic assay; magnetic nanoparticles; signal materials; Gold; Immune system; Magnetic resonance imaging; Magnetic separation; Materials; Nanoparticles; Strips; Assay; Fe3O4 nanoparticles; hepatitis B surface antigen (HBsAg); immunochromatography; signal material;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/TMAG.2014.2324613

Filename

6971700