Four-leaf-clover-shaped immune response chip by using optoelectronic tweezers force

To study the immune system, the natural killer (NK) cell and target cell needs to contact for cell-cell interaction. Therefore, an optoelectronic tweezers (OET) complex optofluidic and four-leaf-clover-shaped (FLCS) microwells, multilayered platform was designed and fabricated to increase interaction. In this study, a novel method uses OET to attract immune cell into the dead zone. The proposed design creates a dynamic observation area with slower flow velocity enabling precise capture of immune cell into the microwells without harm, matching the simulation. Through a simple method, the preliminary data of NK cell and target cell interaction is obtained within 4 hours.