High efficient synthesis of manganese(ii), cobalt(ii) complexes containing lysozyme using reaction area separated micro fluidic device

High-efficient synthesis of Mn(II) and Co(II) complexes containing lysozyme using a reaction area-separated-type microfluidic device was realized. The proposed method has the following advantages compared to the conventional method: reduction of reaction temperature from 40°C to 23°C; remarkable reduction of reaction time from 4 hours to less than 1 second for synthesis of Mn(II) and Co(II) complexes; and atmosphere control (in N₂) of the previous steps is not required because all reagents are isolated from the air. A three-fold yield improvement was achieved in the synthesis of metal complexes containing lysozyme.