DocumentCode
731739
Title
Osmotic erythrocyte lysis for chemical- and label-free impedance cytometry
Author
Winkler, T.E. ; Ben-Yoav, H. ; Kelly, D.L. ; Ghodssi, R.
Author_Institution
Fischell Dept. of Bioeng., Univ. of Maryland, College Park, MD, USA
fYear
2015
fDate
21-25 June 2015
Firstpage
351
Lastpage
354
Abstract
We present the first implementation of osmotic erythrocyte (RBC) lysis for microfluidic leukocyte (WBC) impedance cytometry. The method relies on osmotic pressure differentials to rupture RBC membranes, and detection of WBCs based on changes in impedance. Specifically, we demonstrate an up to 1,200-fold decrease in noise from RBCs in WBC recordings. Our approach enables lab-on-a-chip (LOC)-based WBC counting on whole blood samples without traditionally required chemicals or labels.
Keywords
biomembranes; blood; cellular biophysics; lab-on-a-chip; microfluidics; osmosis; RBC membrane rupture; lab-on-a-chip-based WBC counting; microfluidic leukocyte impedance cytometry; osmotic erythrocyte lysis; osmotic pressure differentials; whole blood samples; MATLAB; Mathematical model; Microsystem integration; impedance cytometry; lab-on-a-chip; microfluidics; osmotic lysis;
fLanguage
English
Publisher
ieee
Conference_Titel
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location
Anchorage, AK
Type
conf
DOI
10.1109/TRANSDUCERS.2015.7180933
Filename
7180933
Link To Document