Using gelatin methacrylate covering and dielectrophoresis force manipulating for lobule-mimicking culture chip in vitro

In vivo, tissue morphology pays an important role in cell single transferring and tissue function increasing. However, maintaining the mimetic cell culture microenvironment in microfluidic chip, several stresses which break the membrane of cells and damage the pattern of the tissue. In the paper, we demonstrate lobule-mimicking culture chip by using gelatin methacrylate (GelMA) covering and dielectrophoresis (DEP) force manipulating. GelMA is a photocrosslinked biocompatible material. The hydrogel material was used in chip for three purposes: precluding the damage between cells or tissue, providing a biological scaffold to keep cell growth, and supplying the nutrient to cells in diffusible situation.