Title :
Single cell manipulation in cell culture media with Self-Locking Optoelectronic Tweezers across a large area
Author :
Yang, Y.J. ; Mao, Y.F. ; Zhu, X.F. ; Shin, K.S. ; Chui, C.O. ; Chiou, P.Y.
Author_Institution :
Dept. of Mech. & Aerosp. Eng., Univ. of California, Los Angeles, Los Angeles, CA, USA
Abstract :
We report a novel Self-Locking Optoelectronic Tweezers (SLOT) for single-cell manipulation in cell culture media across a large area. SLOT overcomes two major technical barriers of conventional optoelectronic tweezers (OET) toward high throughput single-cell manipulation. Its unique lateral, ring-shaped phototransistor design enables manipulation in high conductivity media (1 S/m) and overcomes a fundamental blurry optical pattern issue for single-cell manipulation in large area (> 1 cm2).
Keywords :
cellular biophysics; phototransistors; radiation pressure; OET; SLOT; cell culture media; conventional optoelectronic tweezers; fundamental blurry optical pattern; high conductivity media; ring-shaped phototransistor; self-locking optoelectronic tweezers; single cell manipulation; Biomedical optical imaging; Dark states; Media; Optical buffering; Optical imaging; Optical sensors; Phototransistors; Self-locking optoelectronic tweezers; cell culture medium; large area; single cell;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
DOI :
10.1109/TRANSDUCERS.2015.7181008
