A VACNT integrated handheld device for label-free virus capture, detection and enrichment for genomic analysis

Author

Yeh, Y.T. ; Tang, Y. ; Lu, H. ; Terrones, M. ; Zheng, S.Y.

Author_Institution

Dept. of Biomed. Eng., Pennsylvania State Univ., University Park, PA, USA

fYear

2015

fDate

21-25 June 2015

Firstpage

747

Lastpage

750

Abstract

A portable carbon nanotube (CNT) integrated microfluidic device is presented to capture virus by physical size-based isolation. The device contained porous CNT array made of nitrogen-doped CNT on a fused silica substrate. The biocompatible nitrogen-doped CNT was synthesized by aerosol-assist chemical vapor deposition (AACVD). Avian influenza virus (AIV) H5N8 subtype was captured from chicken swab samples and detected by reverse-transcriptase real-time polymerase chain reaction (RT-qPCR).

Keywords

aerosols; bioMEMS; biochemistry; biomedical equipment; biomedical measurement; carbon nanotubes; chemical vapour deposition; diseases; doping; enzymes; genomics; materials preparation; microfabrication; microfluidics; microorganisms; molecular biophysics; nanofabrication; nanomedicine; nanoporous materials; nitrogen; portable instruments; silicon compounds; AACVD; AIV H5N8 subtype; C:N; CNT integrated microfluidic device; RT-qPCR; SiO₂; VACNT integrated handheld device; aerosol-assist chemical vapor deposition; avian influenza virus capture; biocompatible nitrogen-doped CNT synthesis; chicken swab sample; fused silica substrate; genomic analysis; label-free virus capture; physical size-based isolation; porous CNT array; portable carbon nanotube integrated microfluidic device; reverse-transcriptase real-time polymerase chain reaction; virus detection; virus enrichment; Carbon nanotubes; Furnaces; Iron; Microfluidics; Nitrogen; Silicon compounds; Substrates; Carbon nanotube; microfluidics; point-of-care; virus detection;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181031

Filename

7181031