Title :
Shape Anisotropic Magnetic Particles for high throughput and high efficiecy intracelluar delivery of functional macromolecules
Author :
Lin, M.Y. ; Wu, Y.C. ; Lee, J.A. ; Zhou, J. ; Teitell, M.A. ; Chiou, P.Y.
Author_Institution :
Nat. Appl. Res. Labs., Instrum. Technol. Res. Center, Taiwan
Abstract :
We report a novel intracellular delivery system based on aligning Shape-Anisotropic Magnetic Particles (SAMP) to deliver functional molecules into mammalian cells with high efficiency and high viability. A broad range of molecules (calcein, dextran 3K, dextran 40K, GFP plasmid, SiRNA, and β-lactamase) have been successfully delivered into primary cells with preserved functions. We have achieved high efficiency delivery of enzyme into primary human dermal fibroblast (NHDF), and other large macromolecules (dextran 40K) into mouse cortical neurons.
Keywords :
biomagnetism; cellular biophysics; drug delivery systems; enzymes; magnetic particles; β-lactamase; GFP plasmid; SiRNA; calcein; dextran 3K; dextran 40K; enzyme; functional macromolecules intracellular delivery; mammalian cells; mouse cortical neurons; primary human dermal fibroblast; shape anisotropic magnetic particles; Biochemistry; Fibroblasts; Magnetic particles; Mice; Neurons; Perpendicular magnetic anisotropy; Intracellular delivery; SiRNA; functional macromolecules; shape-anisotropic magnetic particles;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
DOI :
10.1109/TRANSDUCERS.2015.7181064
