In-vivo single cell protein interaction investigation using microfluidic platform

We have developed a microfluidic platform to immobilize single living cells at a position in a microfluidic channel for monitoring cell responses to different stimuli over time. While the cell is trapped, the device maintains control over changing the channel media and flow rate. The captured cell may be released in order to capture another cell and repeat the experiment without disturbing the setup. We have demonstrated the monitoring of an individual yeast cell, in which the captured cell is exposed to α-factor ligand.