Real-time multi-analyte online monitoring of 3d cell cultures by integrated enzyme-based biosensors in hanging drop networks

Author

Misun, P.M. ; Rothe, J. ; Hierlemann, A. ; Frey, O.

Author_Institution

Dept. of Biosyst. Sci. & Eng., ETH Zurich, Basel, Switzerland

fYear

2015

fDate

21-25 June 2015

Firstpage

1625

Lastpage

1628

Abstract

We present the integration of enzyme-based lactate and glucose biosensors into hanging-drop networks. Hangingdrop networks are an ideal microfluidic platform for formation, cultivation, and continuous and long-term observation of 3D microtissues. The implementation of biosensors enables in-situ online monitoring of the effects of different culturing conditions and compound dosages on the microtissues. A hybrid approach including glass sensor modules embedded into a microfluidic polydimethylsiloxane (PDMS)-based chip facilitates system integration. The biosensors enable real-time recording of lactate production and glucose consumption of human colon carcinoma spheroids.

Keywords

bioMEMS; biochemistry; biological tissues; biomedical equipment; biosensors; cellular biophysics; chemical sensors; microfluidics; microsensors; patient monitoring; sugar; 3D cell cultures; 3D microtissues; glass sensor modules; hanging-drop networks; human colon carcinoma spheroids; hybrid approach; in-situ online monitoring; integrated enzyme-based biosensors; lactate production; microfluidic polydimethylsiloxane-based chip; real-time multianalyte online monitoring; Biosensors; Calibration; Electrodes; Glass; Microfluidics; Sugar; Three-dimensional displays; 3D microtissues; Biosensor; Glucose; Hanging-drop network; Lactate; Microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181252

Filename

7181252