Title :
D2O based microfluidics for in vitro ir cell analysis
Author :
Ebrahimifard, R. ; van den Driesche, S. ; Lang, W. ; Vellekoop, M.J.
Author_Institution :
Microsyst. Center Bremen (MCB), Univ. of Bremen, Bremen, Germany
Abstract :
We present the use of D2O (deuterium oxide) as a liquid medium for infrared analysis of biological cells in microfluidic devices. In contradiction to standard buffer liquids, D2O shows a low infrared absorption in the range of 3.3 to 3.6 μm wavelength, which makes it suitable for infrared analysis methods in that range. We illustrate the performance by the measurement of CH2 and CH3 stretch vibrations of phospholipid bilayer of the cell membrane of yeast, in a microfluidic chip.
Keywords :
bioMEMS; biological techniques; biomembranes; cellular biophysics; heavy water; infrared spectroscopy; lab-on-a-chip; lipid bilayers; microfluidics; microorganisms; molecular biophysics; vibrational states; CH2 stretch vibration measurement; CH3 stretch vibration measurement; D2O; D2O based microfluidics; biological cell analysis; cell membrane phospholipid bilayer; deuterium oxide liquid medium; in vitro IR cell analysis; infrared analysis; microfluidic chip; standard buffer liquid; wavelength 3.3 mum to 3.6 mum; wavelength range; yeast; Absorption; Biomembranes; Microfluidics; Semiconductor device measurement; Vibrations; Water; D2O; cells; infrared sensor; microfluidics;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
DOI :
10.1109/TRANSDUCERS.2015.7181268
