Continuous lactate monitoring by microsensors in spheroid 3D tumor cell cultures

Author

Weltin, A. ; Hammer, S. ; Kaminski, Y. ; Klein, S. ; Noor, F. ; Kieninger, J. ; Urban, G.A.

Author_Institution

Dept. of Microsyst. Eng. - IMTEK, Univ. of Freiburg, Freiburg, Germany

fYear

2015

fDate

21-25 June 2015

Firstpage

1695

Lastpage

1698

Abstract

This paper reports the combination of an electrochemical metabolic monitoring system with a spheroid 3D tumor cell culture environment. Precise lactate production from single human hepatocyte spheroids was continuously monitored online over hours by electrochemical microsensors, also in combination with drug exposure. The approach enhances and simplifies the determination of metabolic rates from organotypic 3D cell cultures in drug discovery and toxicology.

Keywords

bioMEMS; biochemistry; biomedical equipment; cellular biophysics; drugs; electrochemical sensors; microsensors; organic compounds; toxicology; tumours; continuous lactate monitoring; drug discovery; drug exposure; drug toxicology; electrochemical metabolic monitoring system; electrochemical microsensor; lactate production; metabolic rate determination; online monitoring; organotypic 3D cell culture; single human hepatocyte spheroid; spheroid 3D tumor cell culture environment; Biosensors; Drugs; Electrodes; Monitoring; Production; Three-dimensional displays; 3D cell culture; lactate; microsensors; monitoring; spheroids; toxicology;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181270

Filename

7181270