Title :
Visualizing mammalian brain area interactions by dual-axis two-photon calcium imaging
Author_Institution :
Depts. of Biol. & Appl. Phys., Stanford Univ., Stanford, CA, USA
Abstract :
Fluorescence Ca2+ imaging enables large-scale recordings of neural activity, but collective dynamics across mammalian brain regions are generally inaccessible within single fields of view. Here we introduce a two-photon microscope possessing two articulated arms that can simultaneously image two brain areas (~0.38 mm2 each), either nearby or distal, using microendoscopes, in awake behaving rodents.
Keywords :
biomedical optical imaging; brain; calcium; endoscopes; fluorescence; neurophysiology; optical microscopy; two-photon processes; articulated arms; awake behaving rodents; collective dynamics; dual-axis two-photon calcium imaging; fluorescence Ca2+ imaging; large-scale recordings; mammalian brain area interaction visualization; microendoscopes; neural activity; two-photon microscope; Biomedical imaging; Biotechnology; Brain; Microscopy; Optical microscopy;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
