Title :
Engineering the next generation of optogenetic reporters to illuminate neuronal activity
Author :
Campbell, Robert E.
Author_Institution :
Dept. of Chem., Univ. of Alberta, Edmonton, AB, Canada
Abstract :
The Campbell group uses protein engineering to develop fluorescent protein-based reporters for cell imaging. I will describe our most recent efforts to engineer an improved generation of reporters for calcium ion, membrane potential, and neurotransmitters.
Keywords :
bioelectric potentials; biomembrane transport; fluorescence; genetics; molecular biophysics; neurophysiology; proteins; Campbell group; calcium ion; cell imaging; fluorescent protein-based reporters; membrane potential; neuronal activity; neurotransmitters; optogenetic reporters; protein engineering; Biological system modeling; Fluorescence; Imaging; Neurotransmitters; Protein engineering; Proteins; Visualization;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
