Title :
Label-free imaging of single neuron activities by stimulated Raman scattering
Author :
Delong Zhang ; Hyeon Jeong Lee ; Pei-Yu Shih ; Drenan, Ryan E. ; Ji-Xin Cheng
Author_Institution :
Weldon Sch. of Biomed. Eng., Purdue Univ., West Lafayette, IN, USA
Abstract :
We report the observation of single neuron activities by spectral focusing stimulated Raman scattering (SRS) microscopy. Changes in SRS signal intensity in live neurons correlate to action potentials indicated by fluorescent calcium indicator.
Keywords :
calcium; cellular biophysics; fluorescence; optical focusing; optical microscopy; stimulated Raman scattering; Ca; SRS signal intensity; fluorescent calcium indicator; label-free imaging; single neuron activities; spectral focusing stimulated Raman scattering microscopy; Calcium; Fluorescence; Image resolution; Microscopy; Neurons; Raman scattering;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
