Wide-field lifetime-based Förster Resonance Energy Transfer in Live animals

Förster Resonance Energy Transfer (FRET) plays an increased role in the drug development pipeline as it allows detecting and monitoring the binding of targeting ligands to receptors as well as receptor dimerization. Herein, we will present our instrumental, theoretical and experimental efforts to establish a new method to perform whole-body FRET imaging based on wide-field time-resolved structured illumination. We will present its application to monitor receptor dimerization/internalization quantitatively in vivo and to specifically discriminate between different Transferrin (Tfn)-targeted systems.