Title :
Maximally informative point spread functions for 3D super-resolution imaging
Author :
Shechtman, Yoav ; Sahl, Steffen J. ; Backer, Adam S. ; Moerner, W.E.
Author_Institution :
Dept. of Chem., Stanford Univ., Stanford, CA, USA
Abstract :
We generate optimal point spread functions (PSFs) for 3D super-resolution imaging, and demonstrate their application in biological conditions. These PSFs exhibit significantly improved localization precision and depth of field over the current state of the art.
Keywords :
biological techniques; biology computing; image resolution; optical transfer function; 3D super-resolution imaging; PSF; biological conditions; depth of field; localization precision; maximally informative point spread functions; optimal point spread functions; Fluorescence; Microscopy; Optical imaging; Spatial resolution; Three-dimensional displays;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
