Title :
Adaptive optics for single molecule switching nanoscopy
Author :
Burke, Daniel ; Patton, Brian ; Huang, Fang ; Bewersdorf, Joerg ; Booth, Martin J.
Author_Institution :
Centre for Neural Circuits & Behaviour, Univ. of Oxford, Oxford, UK
Abstract :
Specimen-induced aberrations frequently affect image quality in high-resolution microscopes. Aberration effects can be even more problematic in super-resolution methods. We show adaptive aberration correction in STORM microscopy of deep cell and tissue specimens.
Keywords :
aberrations; adaptive optics; biological techniques; biological tissues; biology computing; cellular biophysics; image reconstruction; image resolution; optical microscopy; STORM microscopy; adaptive aberration correction; adaptive optics; deep cell specimens; high-resolution microscopes; image quality; single molecule switching nanoscopy; specimen-induced aberrations; stochastic optical reconstruction microscopy; superresolution methods; tissue specimens; Adaptive optics; Biomedical optical imaging; Image resolution; Microscopy; Optical imaging; Optical microscopy; Storms;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
