Investigation of electroporation effect on HT29 cell lines adhesion properties

Electroporation is a method of permeabilizing a living cell membrane with high electric field intensity of short duration. This study investigates the effect of electroporation on colon cell line, HT29 adhesion Property as a preliminary study of the desire to design a potential application of electroporation on wound healing. The study found out that when HT29 cell lines were electroporated with 600 V/cm and 500 μs pulse duration it attached to the monolayer after 12 minutes of Electroporation, and covers a distance of 38.7 μm after one hour. While the non-electroporated cell line for control under the same condition attached to monolayer after 37minutes from floating state and covers a distance of 31.53 μm after 55 minutes. The result showed that electroporated HT29 cell line can attached faster and spread wider than non-electroporated HT29 cell line that can be used to facilitate cell migration for wound healing application.