Efficacy Evaluation of AAV2-EPO to Diabetic Retinopathy Based on Molecular Hyperspectral Imaging System

AAV2-EPO (Adeno-associated virus type 2-mediated EPO) efficacy to early diabetic retinopathy (DR) of experimental rats was studied by molecular hyper spectral imaging system (MHIS). Molecular hyper spectral images of rat retinal tissue slice were collected in the normal control group N, the diabetic group D, the diabetes treatment group by AAV2-EPO (low dose group E1, middle dose group E2, high dose group E3). Raw data was pretreated by the obtained data model. By qualitative analysis of images and spectra, it were proves that AAV2-EPO has some efficacy to early diabetic retinopathy of rats. The experimental results show that AAV2-EPO treatment with middle dose can be most effective in reducing nerve cells apoptosis of retinal outer nuclear layer and have a certain effect to DR. Thus MHIS can help researchers exploring pathogenesis, causes of blindness and drug development of DR.