A multimicroelectrode system composed of independent glass micropipettes with an eccentric tip structure for simultaneous intracellular recording

Author

Saburi, Masahisa ; Yamada, Masahiro ; Shigematsu, Yukifumi

Author_Institution

Electrotech. Lab., Agency of Ind. Sci. & Technol., Ibaraki, Japan

Volume

39

Issue

6

fYear

1992

fDate

6/1/1992 12:00:00 AM

Firstpage

656

Lastpage

658

Abstract

A glass multimicroelectrode system for simultaneous intracellular recordings in which four pieces of micropipettes with rectangular cross section are arranged in a square compartment is described. Techniques are outlined for fabricating such an eccentric micropipette. An example of simultaneous recordings of intracellular membrane potentials in the carp retina is demonstrated. A technique which would allow researchers to record from three or four neurons simultaneously is presented.

Keywords

bioelectric potentials; biological techniques and instruments; cellular biophysics; microelectrodes; neurophysiology; carp retina; eccentric micropipette fabrication techniques; eccentric tip structure; independent glass micropipettes; intracellular membrane potentials; multimicroelectrode system; rectangular cross section; simultaneous intracellular recording; Biomedical electrodes; Biomembranes; Extracellular; Fabrication; Glass; Magnetic recording; Neurons; Optical arrays; Optical recording; Retina; Animals; Carps; Equipment Design; Glass; Membrane Potentials; Microelectrodes; Nerve Net; Neurophysiology; Retinaldehyde;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/10.141205

Filename

141205