DocumentCode :
757372
Title :
Antibody Immobilization on Functional Monolayers Using a Quartz Crystal Microbalance
Author :
Aizawa, Hidenobu ; Gokita, Yasutoshi ; Park, Jong-Won ; Yoshimi, Yasuo ; Kurosawa, Shigeru
Author_Institution :
Nat. Inst. of Adv. Ind. Sci. & Technol., Ibaraki
Volume :
6
Issue :
5
fYear :
2006
Firstpage :
1052
Lastpage :
1056
Abstract :
This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4´-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer
Keywords :
Langmuir-Blodgett films; biosensors; chemical sensors; chemical variables measurement; microbalances; organic compounds; quartz; 11-mercaptoundecanoic acid; 2-aminoethanethiol; 4,4´-dithiodibutyric acid; CRP detection; Langmuir isotherm equation; MUA monolayer; anti-C-reactive protein; anti-CRP antibodies; antibody immobilization; functional monolayers; gold surface; immunosensor; monoclonal antibody; quartz crystal microbalance; Biochemical analysis; Biomembranes; Calibration; Coatings; Equations; Gold; Immune system; Materials science and technology; Proteins; Solids; Anti-C-reactive protein (CRP) antibody; CRP detection; functional monolayer; immunosensor; quartz crystal microbalance (QCM);
fLanguage :
English
Journal_Title :
Sensors Journal, IEEE
Publisher :
ieee
ISSN :
1530-437X
Type :
jour
DOI :
10.1109/JSEN.2006.881368
Filename :
1703460
Link To Document :
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