The mechanisms of high gradient magnetic separation of human blood and bone marrow

Author

Richards, Adrian J. ; Roath, O. Stuart ; Smith, Richard J S ; Watson, James H P

Author_Institution

Inst. of Cryogenics, Southampton Univ., UK

Volume

32

Issue

2

fYear

1996

fDate

3/1/1996 12:00:00 AM

Firstpage

459

Lastpage

470

Abstract

Novel HGMS filters have been used to separate dextran-iron labeled human blood and bone marrow cells. Tetrameric antibody complexes for CD8 and CD34 were used for the magnetic labeling. Normalized wire array and process parameters were used to describe the recovery experimental result. Purity was examined using nonmagnetic collision mechanisms. Additional construction details of the filters are given to what has been published previously. A number of experiments with different separation conditions are described which may be of use for difficult biological separation applications

Keywords

biological techniques; biomagnetism; blood; bone; cellular biophysics; filtration; magnetic separation; CD34; CD8; HGMS filters; biological separation; bone marrow; dextran-iron; high gradient magnetic separation; human blood; magnetic labeling; nonmagnetic collisions; process parameters; purity; recovery; tetrameric antibody complexes; wire array; Australia; Blood; Bones; Filters; Humans; Magnetic separation; Physics; Saturation magnetization; Separation processes; Wire;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/20.486533

Filename

486533